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DEPARTMENT OF HEALTH AND HUMAN SERVICES - PUBLIC HEALTH SERVICE
NOTICE OF INTRAMURAL RESEARCH PROJECT
PROJECT NUMBER
ZOl AI 00338-07 LID
PERIOD COVERED
October 1, 1987 to September 30, 1988
TITLE OF PROJECT (80 characters Metoprolol Toprol Xl or less. Title must tit on one //ne Between me borders )
Molecular Cloning of Rotavirus Genes
PRINCIPAL Toprol Recall INVESTIGATOR Lopressor Toprol Xl MAN-YEARS:
PROFESSIONAL:
CHECK APPROPRIATE BOX(ES)
D (a) Human subjects D (b) Human tissues H (c) Neither
D (a1) Minors
n (a2) Interviews
SUMMARY OF WORK (Use Cheap Toprol standarO unreduced type. Do not exceed the space provided.)
The analysis of cloned rotavirus genes will greatly enchance our knowledge of the molecular
biology of rotavirus. In addition to identification of important regulatory signals (promoters,
ribosomal binding sites, etc.), Toprol Xl 25mg analysis of cloned cDNA can yield information concerning amino acid
sequences of structural proteins.
Expression of rotavirus antigens by insertion of cloned DNA from VP7 or VP3 into various
vectors may provide us with safer RV vaccines. Although rotavirus cDNA clones Toprol Xl 50 representing VP7
have been inserted into bacterial and vaccinia expression vectors, the proteins expressed have not
been highly immunogenic. It is possible, however, that insertion of the VP3 protein gene into
such vectors will result in Toprol Online the expression of a more antigenic protein. VP3 and VP7 clones are
being engineered for insertion into adenovirus and bacterial expression vectors which are currently
under development.
We are currentiy attempting to transfect rotavirus ss RNA from a given serotype into cells
infected with a rotavirus strain of a different serotype in an attempt to recover (under selective
pressure) rotavirus reassortants containing the transfected gene. If these experiments yield
positive results it will be possible to attempt the rescue of rotavirus modified by in vitro
mutagenesis of cloned DNA. Success in developing this methodology will Toprol Price also allow Buy Toprol Online us to probe
the function of certain areas of the rotavirus genome (i.e. serotype specific regions in the VP7
gene, cleavage region in the fourth gene, etc.) and could be of considerable help in constructing
cross-reactive or further attenuated rotavirus vaccine candidates.
PHS 6040 (Rev 1/84) 11-67 0POBi«i«
pooject number
department of health and human services - public health service
NOTICE OF INTRAMURAL RESEARCH PROJECT ZOl AI 00339-07 LID
PEROO COVERED
October 1. 1987 to September 30. Buy Toprol Xl 1988
TITLE 0^ PROJECT BO crvcnr% Toprol Cost which was a "supershort"
cleccropherotype, was found to belong to serotype 6 (prototype bovine rotavirus NCDV strain
which has a "long" clectropherotype) by PRN assay. Bovine rotavirus strain B223 was shown, by a
one-way cross-neutralization assay, to be distinct from any of the 9 established serotypes.
11-68
aPO tiA-ait
DEPARTMENT OF HEALTH AND HUMAN SERVICES - PUBLIC HEALTH SERVICE
NOTICE OF INTRAMURAL RESEARCH PROJECT
PROJECT NUMBER
ZOl AI 00340-07 LID
PERIOD COVERED
October 1, 1987 to September 30, Order Toprol Xl 1988
TITLE OF PROJECT (80 characters or less. Title must tit on one Toprol 100 Mg line between the borders )
Experimental Studies in Animals with Various Rotaviruses and Their Reassortants.
PRINCIPAL INVESTIGATOR (List other professional personnel below the Principal Investigator ) (Name, title, laboratory, and institute affiliation)
PI: Yasutaka Hoshino, D.V.M. Visit. Scientist LID, NIAID
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